Human IgE Mouse Monoclonal Antibody [Clone ID: BE5]

CAT#: SM3061P

Human IgE mouse monoclonal antibody, clone BE5, Purified

Product Images

Figure 2: staining of IgE stimulated sample

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  Surface staining of IgE in human peripheral blood lymphocytes with anti-IgE (BE5) purified, GAM-APC.

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  Flow Cytometry analysis of basophil activation upon stimulation of normal (heparin-treated) whole blood with combination of IL-3 and Goat anti-IgE polyclonal antibody. Combination of anti-human IgE (BE5) FITC and anti-human CD63 (MEM-259) PE-Dyomics 647 ([SM3035P6]) was used (analysis in basophil window). Figure 1: staining of non-stimulated (control) sample.

Specifications

Product Data

• Clone Name: BE5
• Applications: ELISA, FC
• Recommended Dilution: ELISA.
  Flow Cytometry: 5-10 µg/ml.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified human IgE
• Specificity: The antibody reacts with human IgE; it recognizes an epitope different from the ones recognized by clone 4G7 and clone 4H10 antibodies to IgE.
• Formulation: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
  State: Purified
  State: Liquid Ig fraction
• Form: Purified
• Concentration: lot specific
• Purification: Precipitation methods and ion exchange chromatography; purity: > 95% (by SDS-PAGE)
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.
  DO NOT FREEZE!