Chicken IgG / Chicken IgY (light chain) Mouse Monoclonal Antibody [Clone ID: 14A3]
| Product Data | |
| Clone Name | 14A3 |
|---|---|
| Application | ELISA, FC, WB |
| Recommended Dilution | Flow Cytometry. ELISA. Western Blot. |
| Reactivity | Duck |
| Antibody Host | Mouse |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Immunogen | Purified Pekin duck yolk IgY. Spleen cells from immunised Balb/c mice were fused with cells of the SP2/0 mouse myeloma cell line. |
| Specificity | This antibody recognises the light chain of duck immunoglobulin. Double-staining with clone 14A3 and duck CD8 alpha clone Du CD8-1 (product code MCA2479 ), revealed the presence of a CD8high /14A3- cytotoxic T cell population and a CD8low/14A3+ B cell population in duck spleen and also revealed a high percentage of CD8+ /14A3+ cells in duck bursa. The recognition of B cells by 14A3 was confirmed using a recently developed Flag-tagged chicken B cell marker, known as chBAFF-Flag (B cell activating factor). This antibody recognises IgA in Pekin duck bile and can be used as a marker to identify B cells in both Pekin and Muscovy duck and geese studies. This antibody detects a band of approximately 25 kDa in duck cell lysates in Western blotting. |
| Buffer | PBS containing 0.09% Sodium Azide State: Purified State: Liquid purified IgG |
| Presentation State | Purified |
| Concentration | lot specific |
| Purification | Affinity chromatography on Protein G |
| Conjugation | Unconjugated |
| Storage | Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. |
| Shipping | Blue Ice |
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