Mouse IgM (Mu chain specific), F(ab)2 Fragment Goat Polyclonal Antibody

SKU R1408TR

Mouse IgM (Mu chain specific), F(ab)2 Fragment goat polyclonal antibody, Texas Red

Product Data

• Recommended Dilution: Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.; Recommended Dilutions:; FLISA: 1/10,000-1/50,000.; IF Microscopy: 1/1,000-1/5,000.; Flow Cytometry: 1/500-1/2,500.
• Reactivity: Mouse
• Antibody Host: Goat
• Clonality: Polyclonal
• Immunogen: Mouse IgM mu chain specific.
• Specificity: This product was prepared from monospecific antiserum by Immunoaffinity Chromatography using Mouse IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.; Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgM and Mouse Serum.; No reaction was observed against anti-Pepsin or anti-Goat IgG F(c). Specificity was confirmed by ELISA at less than 1% cross reactivity against other mouse heavy or light chain isotypes.
• Buffer: 0.02M Sodium Phosphate, 0.15M Sodium Chloride, pH 7.2 with 0.01% (w/v) Sodium Azide as preservative and 10 mg/ml BSA (IgG and Protease free) as stabilizer.; Label: Texas Red; State: Lyophilized F(ab')2 fragments.; Label: -- Sulfonyl Chloride (Molecular Weight 625 daltons); Absorption emission: 596 nm / 620 nm; Molar radio: 1.7 moles Texas Red(TM) per mole of Goat IgG F(ab')2.
• Reconstitution Method: Restore with 1 ml of deionized water (or equivalent).
• Concentration: lot specific
• Purification: Immunoaffinity Chromatography.
• Conjugation: Texas Red
• Storage: Store vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature.; This antibody is stable for one month at 2-8°C as an undiluted liquid.; Dilute only prior to immediate use.; Avoid repeated freezing and thawing.
• Shipping: Ambient