Mouse IgG (Fc specific), F(ab)2 Fragment, adsorbed Goat Polyclonal Antibody

SKU R1406R

Mouse IgG (Fc specific), F(ab)2 Fragment, adsorbed goat polyclonal antibody, PE

Product Data

• Application: IF
• Recommended Dilution: Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 10⁶ cells in Flow Cytometry is approximately 1.0 µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining. As a general guideline dilutions of 1/100 to 1/250 should be suitable for most applications.; Recommended Dilutions: ; Immunofluorescence: 1/100-1/250.; Flow Cytometry: 1/100-1/250.
• Reactivity: Mouse
• Antibody Host: Goat
• Clonality: Polyclonal
• Immunogen: Human IgG F(c) fragment.
• Specificity: Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Goat Serum, Mouse IgG, Mouse IgG F(c) and Mouse Serum.; No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Mouse IgG F(ab')2 or Bovine, Horse or Human Serum Proteins.
• Buffer: 0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2; Label: PE; State: Lyophilized F(ab')2 fragments; Stabilizer: 10 mg/ml BSA (IgG and Protease free); Preservative: 0.01% (w/v) Sodium Azide; Label: R-Phycoerythrin ( (Molecular Weight 240,000 daltons); Absorption emission: 488 nm / 575 nm
• Reconstitution Method: Restore with 1.0 ml deionized Water (or equivalent).
• Concentration: lot specific
• Purification: Immunoaffinity Chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
• Conjugation: PE
• Storage: This antibody is stable at 2-8°C as an undiluted liquid.; DO NOT FREEZE!; Restore with 1.0 ml of deionized water (or equivalent).; Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis.; Dilute only prior to immediate use.; Avoid repeated freezing and thawing.
• Shipping: Ambient