Goat IgG (H+L chain), F(ab)2 Fragment, adsorbed Rabbit Polyclonal Antibody

SKU R1392B

Goat IgG (H+L chain), F(ab)2 Fragment, adsorbed rabbit polyclonal antibody, Biotin

Product Data

• Recommended Dilution: Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.; Recommended Dilutions: This product has been assayed against 1.0 ug of Goat IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.; A working dilution of 1:40,000 to 1:200,000 is suggested for this product.; The following general recommendations are suggested as starting dilutions for:; Immunoblotting: 1:10,000,; Enzyme Immunohisto-chemistry on tissue sections: 1:500-1:5,000.; Flow cytometry and fluorescence Immunohisto/cytochemistry: 1:200-1:1,000.
• Reactivity: Goat
• Antibody Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Goat IgG whole molecule.
• Specificity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.; Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, Goat IgG and Goat Serum.; No reaction was observed against Human Serum Proteins.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free as stabilizer and 0.05% (w/v) Sodium Azide as preservative.; Label: Biotin; State: Lyophilized F(ab')2 fragments.; Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC); Molar radio: 10-20 BAC molecules per Rabbit IgG Fab molecule.
• Reconstitution Method: Restore with 0.5 ml of deionized water (or equivalent).
• Concentration: lot specific
• Purification: Immunoaffinity chromatography.
• Conjugation: Biotin
• Storage: Store vial at 4°C prior to restoration. For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20°C or below.; Centrifuge product if not completely clear after standing at room temperature.; This antibody is stable for one month at 4°C as an undiluted liquid.; Dilute only prior to immediate use.; Avoid cycles of freezing and thawing.
• Shipping: Ambient