Human IgG (H+L chain), Fab Fragment Goat Polyclonal Antibody

SKU R1388F

Human IgG (H+L chain), Fab Fragment goat polyclonal antibody, FITC

Product Data

• Recommended Dilution: Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.; Recommended Dilutions:; FLISA: 1/10,000-1/50,000.; Flow Cytometry: 1/500-1/2,500.; IF Microscopy: 1/1,000-1/5,000.
• Reactivity: Human
• Antibody Host: Goat
• Clonality: Polyclonal
• Immunogen: Human IgG whole molecule
• Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein and anti-Goat Serum.; No reaction was observed against anti-Papain or anti-Goat IgG F(c).
• Buffer: 0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2, containing 10 mg/ml BSA (IgG and Protease free) and 0.01% (w/v) Sodium Azide; Label: FITC; State: Lyophilized Fab fragment; Label: Fluorescein isothiocyanate (Molecular Weight 390 daltons); Absorption emission: 495 nm / 528 nm; Molar radio: 4.1 moles FITC per mole of Goat IgG Fab
• Reconstitution Method: Restore with 1.0 ml of deionized water or equivalent.
• Concentration: lot specific
• Purification: Immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation
• Conjugation: FITC
• Storage: Store vial at 2-8°C prior to restoration.; Restore with deionized water (or equivalent); centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8°C as an undiluted liquid.; For extended storage aliquot contents and freeze at -20°C or below. Avoid repeated freezing and thawing.; Dilute only prior to immediate use.
• Shipping: Ambient