Porcine IgG F(ab)2 specific Rabbit Polyclonal Antibody

SKU R1386F

Porcine IgG F(ab)2 specific rabbit polyclonal antibody, FITC

Product Data

• Recommended Dilution: Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.; Recommended Dilution(s):; Flow Cytometry: 1:2,000-1:10,000.; IF Microscopy: 1:500-1:2,500.
• Reactivity: Pig
• Antibody Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Swine IgG F(ab')2 fragment.
• Specificity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Swine IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.; Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rabbit Serum, Swine IgG, Swine IgG F(ab')2 and Swine Serum.; No reaction was observed against Swine IgG F(c).
• Buffer: 0.02 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, with 0.01% (w/v) sodium azide as preservative and 10 mg/ml Bovine Serum Albumin (BSA; IgG and Protease free) as stabilizer.; Label: FITC; State: Lyophilized purified Ig fraction.; Label: Fluorescein isothiocyanate (Molecular Weight 390 daltons); Absorption emission: 495 nm / 528 nm; Molar radio: 2.2 moles FITC per mole of Rabbit IgG.
• Reconstitution Method: Restore with 1.0 ml of deionized water (or equivalent).
• Purification: Immunoaffinity chromatography.
• Conjugation: FITC
• Storage: Store vial at 4°C prior to restoration. For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20°C or below.; Centrifuge product if not completely clear after standing at room temperature.; This antibody is stable for one month at 4°C as an undiluted liquid.; Dilute only prior to immediate use.; Avoid cycles of freezing and thawing.
• Shipping: Ambient