Porcine IgG F(ab)2 specific Rabbit Polyclonal Antibody

SKU R1386AP

Porcine IgG F(ab)2 specific rabbit polyclonal antibody, AP

Product Data

• Recommended Dilution: Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.; Recommended Dilutions:; ELISA: 1:1,000-1:3,000.; Western Blot: 1:500-1:2,000.; Immunohistochemistry: 1:200-1:1,000.
• Reactivity: Pig
• Antibody Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Swine IgG F(ab')2 fragment.
• Specificity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Swine IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.; Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Swine IgG, Swine IgG F(ab') 2 and Swine Serum.; No reaction was observed against Swine IgG F(c).
• Buffer: 0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0 with 10 mg/ml Bovine Serum Albumin (BSA) IgG; Label: AP; State: Lyophilized purified Ig fraction.; Label: Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
• Reconstitution Method: Restore with 0.5 ml of deionized water (or equivalent).
• Concentration: lot specific
• Purification: Immunoaffinity chromatography.
• Conjugation: AP
• Storage: Store the antibody (undiluted) at 2-8°C before opening.; DO NOT FREEZE!; Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.; Dilute only prior to immediate use.
• Shipping: Ambient