Sheep IgG F(ab)2 specific (adsorbed) Rabbit Polyclonal Antibody

CAT#: R1383T

Sheep IgG F(ab)2 specific (adsorbed) rabbit polyclonal antibody, TRITC

Specifications

Product Data

• Recommended Dilution: Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
• Reactivities: Sheep
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Sheep IgG F(ab')2 fragment.
• Specificity: This product was prepared from monospecific antiserum by Immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Sheep IgG, Sheep IgG F(ab')2 and Sheep Serum.
  No reaction was observed against Sheep IgG F(c) or Human Serum Proteins.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 10 mg/ml Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer and 0.01% (w/v) Sodium Azide as preservative.
  Label: TRITC
  State: Lyophilized purified Ig fraction.
  Label: Tetramethylrhodamine isothiocyanante ; Molecular Weight 444 daltons
  Absorption emission: 550 nm / 570 nm
  Molar radio: 2.6 moles TRITC per mole of Rabbit IgG.
• Reconstitution Method: Restore with 0.5 ml of deionized water (or equivalent).
• Concentration: lot specific
• Purification: Immunoaffinity chromatography.
• Conjugation: TRITC
• Storage: Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature.
  This product is stable for one month at 2-8°C as an undiluted liquid.
  Dilute only prior to immediate use.
  Avoid cycles of freezing and thawing.