Sheep IgG (H+L chain), adsorbed Rabbit Polyclonal Antibody

SKU R1379F

Sheep IgG (H+L chain), adsorbed rabbit polyclonal antibody, FITC

Product Data

• Recommended Dilution: Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
• Reactivity: Sheep
• Antibody Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Sheep IgG whole molecule
• Specificity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.; Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rabbit Serum, Sheep IgG and Sheep Serum. No reaction was observed against Human Serum Proteins.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01% (w/v) Sodium Azide as preservative; Stabilizer: 10 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free; Label: FITC; State: Lyophilized Ig fraction; Label: Fluorescein isothiocyanate (Molecular Weight 390 daltons); Absorption emission: 495nm / 528nm; Molar radio: 4.9 moles FITC per mole of Rabbit IgG
• Reconstitution Method: Restore with 1.0 ml of deionized water or equivalent.
• Concentration: lot specific
• Purification: Immunoaffinity chromatography
• Conjugation: FITC
• Storage: Store vial at 2-8° C prior to restoration. Restore with deionized water (or equivalent); centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid.; For extended storage add glycerol to 50% and then aliquot contents and freeze at -20° C or below.; Avoid cycles of freezing and thawing. Dilute only prior to immediate use.
• Shipping: Ambient