Rat IgG (H+L chain, adsorbed) Goat Polyclonal Antibody

SKU R1378HRP

Rat IgG (H+L chain, adsorbed) goat polyclonal antibody, HRP

Product Data

• Application: ELISA, IHC, WB
• Recommended Dilution: Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.; Recommended Dilutions:; ELISA: 1/100,000.; Western blot: 1/2,000-1/10,000.; Immunohistochemistry: 1/500-1/1,500.
• Reactivity: Rat
• Antibody Host: Goat
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Rat IgG whole molecules.
• Specificity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.; Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rat IgG and Rat Serum.; No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit and Sheep Serum Proteins.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, with 10 mg/ml Bovine Serum Albumin (BSA) (IgG and Protease free) as stabilizer and 0.01% (w/v) Gentamicin Sulfate as preservative.; Label: HRP; State: Lyophilized purified Ig fraction.; Label: Horseradish Peroxidase
• Reconstitution Method: Restore with 1.0 ml of deionized water (or equivalent).
• Concentration: lot specific
• Purification: Immunoaffinity chromatography.
• Conjugation: HRP
• Storage: Store vial at 4°C prior to restoration. For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20°C or below.; Centrifuge product if not completely clear after standing at room temperature.; This antibody is stable for one month at 4°C as an undiluted liquid.; Dilute only prior to immediate use.; Avoid cycles of freezing and thawing.
• Shipping: Ambient