Rat IgM (Mu chain specific) Rabbit Polyclonal Antibody

SKU R1375HRP

Rat IgM (Mu chain specific) rabbit polyclonal antibody, HRP

Product Data

• Recommended Dilution: Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.; Recommended Dilutions:; ELISA: 1/10,000-1/50,000.; Western Blot: 1/1,000-1/5,000.; Immunohistochemistry: 1/500-1/2,500.
• Reactivity: Rat
• Antibody Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Rat IgM mu heavy chain.
• Specificity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.; Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Rat IgM and Rat Serum.; No reaction was observed against Rat IgA or Rat IgG.; ELISA was used to confirm specificity at less than 1% cross reactivity against other rat heavy or light chain isotypes.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free as stabilizer and 0.01% (w/v) Gentamicin Sulfate as preservative.; Label: HRP; State: Lyophilized purified Ig fraction.; Label: Horseradish Peroxidase
• Reconstitution Method: Restore with 1.0 ml of deionized water (or equivalent).
• Concentration: lot specific
• Purification: Immunoaffinity chromatography.
• Conjugation: HRP
• Storage: Store vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature.; This antibody is stable for one month at 2-8°C as an undiluted liquid.; Dilute only prior to immediate use.; Avoid repeated freezing and thawing.
• Shipping: Ambient