Goat IgG (F(ab)2 specific) Rabbit Polyclonal Antibody

SKU R1320F

Goat IgG (F(ab)2 specific) rabbit polyclonal antibody, FITC

Product Data

• Recommended Dilution: Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
• Reactivity: Goat
• Antibody Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Goat IgG F(ab')2 fragment.
• Specificity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.; Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rabbit Serum, Goat IgG, Goat IgG F(ab')2 and Goat Serum.; No reaction was observed against Goat IgG F(c).
• Buffer: 0.01 M Sodium Phosphate, 0.14 M Sodium Chloride, pH 7.4 with 10 mg/ml Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer and 0.01% (w/v) Thimerisol as preservative.; Label: FITC; State: Lyophilized purified Ig fraction.; Label: Diamino-triazinylaminofluorescein (DTAF; Molecular Weight 530 daltons). DTAF has identical fluorescence properties as; Absorption emission: 495 nm / 528 nm; Molar radio: 3.0 moles DTAF per mole of Rabbit IgG.
• Reconstitution Method: Restore with 1.0 ml of deionized water (or equivalent).
• Concentration: 2.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity chromatography.
• Conjugation: FITC
• Storage: Store vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature.; This antibody is stable for one month at 2-8°C as an undiluted liquid.; Dilute only prior to immediate use.; Avoid repeated freezing and thawing.
• Shipping: Ambient