Goat IgG (H+L chain), adsorbed Rabbit Polyclonal Antibody

SKU R1318T

Goat IgG (H+L chain), adsorbed rabbit polyclonal antibody, TRITC

Product Data

• Application: ELISA, FC, IF
• Recommended Dilution: Suitable for ELISA, Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.; Recommended Dilutions:; ELISA: 1/10,000 - 1/50,000.; IF Microscopy: 1/1000 - 1/5000.
• Reactivity: Goat
• Antibody Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Goat IgG whole molecule.
• Specificity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.; Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Goat IgG, and Goat Serum.; No reaction was observed against Human Serum Proteins.
• Buffer: 0. 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, with 0.01% (w/v) Sodium Azide as preservative and 10 mg/ml Bovine Serum Albumin (BSA) (IgG and Protease free) as stabilizer.; Label: TRITC; State: Lyophilized purified Ig fraction.; Label: Tetramethylrhodamine isothiocyanante (Molecular Weight 444 daltons); Absorption emission: 550 nm / 570 nm; Molar radio: 2.8 moles TRITC per mole of Rabbit IgG.
• Reconstitution Method: Restore with 1.0 ml of deionized water (or equivalent).
• Concentration: lot specific
• Purification: Immunoaffinity chromatography.
• Conjugation: TRITC
• Storage: Store vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature.; This antibody is stable for one month at 2-8°C as an undiluted liquid.; Dilute only prior to immediate use.; Avoid repeated freezing and thawing.
• Shipping: Ambient