Goat IgG (H+L chain), adsorbed Rabbit Polyclonal Antibody

SKU R1318HRP

Goat IgG (H+L chain), adsorbed rabbit polyclonal antibody, HRP

Product Data

• Recommended Dilution: Suitable for immunoblotting (Western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.; Recommended dilutions; ELISA: 1/10,000-1/50,000.; Western blot: 1/1,000-1/5,000.; Immunohistochemistry: 1/500-1/2,500.
• Reactivity: Goat
• Antibody Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Goat IgG whole molecule
• Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Goat IgG and Goat Serum.; No reaction was observed against Human Serum Proteins.
• Buffer: 0.02 MPotassium Phosphate, 0.15M Sodium Chloride, pH 7.2, with 0.01% (w/v) Gentamicin Sulfate as preservative and 10 mg/ml BSA (IgG and Protease free) as stabilizer.; Label: HRP; State: Lyophilized purified Ig fraction; Label: Horseradish Peroxidase
• Reconstitution Method: Restore with 1.0 ml deionized water or equivalent.
• Concentration: lot specific
• Purification: Immunoaffinity Chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities
• Conjugation: HRP
• Storage: Store vial at 2-8°C prior to restoration.; Restore with deionized water (or equivalent); centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8°C as an undiluted liquid.; For extended storage aliquot contents and freeze at -20°C or below. Avoid repeated freezing and thawing.; Dilute only prior to immediate use.
• Shipping: Ambient