Goat IgG (H+L chain), adsorbed Rabbit Polyclonal Antibody

SKU R1318F

Goat IgG (H+L chain), adsorbed rabbit polyclonal antibody, FITC

Product Data

• Recommended Dilution: Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.; Recommended Dilutions:; FLISA: 1/10,000-1/50,000.; Flow Cytometry: 1/500-1/2,500.; IF Microscopy: 1/1,000-1/5,000.
• Reactivity: Goat
• Antibody Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Goat IgG whole molecule.
• Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rabbit Serum, Goat IgG and Goat Serum.; No reaction was observed against Human Serum Proteins.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2; Label: FITC; State: Lyophilized purified Ig fraction.; Stabilizer: 10 mg/ml BSA (IgG and Protease free); Preservative: 0.01% (w/v) Sodium Azide; Label: Fluorescein isothiocyanate (Molecular Weight 390 daltons); Absorption emission: 495 nm / 528 nm; Molar radio: 2.8 moles FITC per mole of Rabbit IgG.
• Reconstitution Method: Restore with 1.0 ml of deionized water (or equivalent).
• Concentration: lot specific
• Purification: Immunoaffinity Chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
• Conjugation: FITC
• Storage: Store the antibody undiluted at 2-8°C, reconstitute and store at 2-8°C for one month or at -20°C for longer.; For extended storage reconstitute product with 50% Glycerol instead of water and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature.; Avoid repeated freezing and thawing.
• Shipping: Ambient