Goat IgG (H+L chain) Rabbit Polyclonal Antibody
CAT#: R1317HRP
Goat IgG (H+L chain) rabbit polyclonal antibody, HRP
Conjugation: Unconjugated Biotin FITC
Specifications
Product Data | |
Applications | ELISA, IHC, WB |
Recommended Dilution | ELISA: 1/50,000-1/100,000. Western Blot: 1/5,000-1/20,000. Immunohistochemistry: 1/1,000-1/5,000. Note: HRP Secondary antibody reagents are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. |
Reactivities | Goat |
Host | Rabbit |
Clonality | Polyclonal |
Immunogen | Goat IgG whole molecule. |
Specificity | This product was prepared from monospecific antiserum by Immunoaffinity Chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Goat IgG, and Goat serum. |
Formulation | 0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2 Label: HRP State: Lyophilized purified Ig fraction Stabilizer: 10 mg/ml BSA (IgG and Protease free) Preservative: 0.01% (w/v) Gentamicin Sulfate Label: Horseradish Peroxidase |
Reconstitution Method | Restore with 1.0 ml of deionized water (or equivalent). |
Concentration | 2.0 mg/ml (by UV absorbance at 280 nm) |
Purification | Immunoaffinity Chromatography |
Conjugation | HRP |
Storage | Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. |
Documents
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FAQs |
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