Canine IgG (F(ab)2 specific) Rabbit Polyclonal Antibody

SKU R1312AP

Canine IgG (F(ab)2 specific) rabbit polyclonal antibody, AP

Product Data

• Recommended Dilution: Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.; Recommended Dilutions:; ELISA: 1/2,000-1/10,000.; Western Blot: 1/500-1/2,500.; Immunohistochemistry: 1/200-1/1,000.
• Reactivity: Dog
• Antibody Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Dog IgG F(ab')2 fragment.
• Specificity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Dog IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.; Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Dog IgG, Dog IgG F(ab')2 and Dog Serum.; No reaction was observed against Dog IgG F(c).
• Buffer: Format: This antibody is an IgG fraction and was lyophilised from 0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0 with 10 mg/ml Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer and 0.01% (w/v) sodium azide as preservative.; Label: AP; State: Liquid (sterile filtered) purified Ig fraction.; Label: Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
• Concentration: lot specific
• Purification: Immunoaffinity chromatography.
• Conjugation: AP
• Storage: Store the antibody undiluted at 2-8°C.; DO NOT FREEZE!; Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.; Dilute only prior to immediate use.
• Shipping: Ambient