Feline IgG F(ab')2 specific Rabbit Polyclonal Antibody

SKU R1309T

Feline IgG F(ab')2 specific rabbit polyclonal antibody, TRITC

Product Data

• Recommended Dilution: Suitable for Immunomicroscopy (1/500-1/2,500) and Flow cytometry (1/2,000-1/10,000) or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
• Reactivity: Feline
• Antibody Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Cat IgG F(ab’)2 fragment.
• Specificity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Cat IgG coupled to agarose beads.; Assay by immunoelectrophoresis resulted in a single precipitin arc against anti- Rabbit Serum, Cat IgG, Cat IgG F(ab')2 and Cat Serum.; No reaction was observed against Cat IgG F(c).
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with Polyethylene Glycol (PEG-8000) as stabilizer and 0.01% (w/v) sodium azide as preservative.; Label: TRITC; State: Lyophilized purified Ig fraction.; Label: Tetramethylrhodamine isothiocyanante (Molecular Weight 444 daltons); Absorption emission: 550 nm / 570 nm; Molar radio: 2.3 moles TRITC per mole of Rabbit IgG.
• Reconstitution Method: Restore with 1.0 ml of deionized water (or equivalent).
• Concentration: 2.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity chromatography.
• Conjugation: TRITC
• Storage: Store vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature.; This antibody is stable for one month at 2-8°C as an undiluted liquid.; Dilute only prior to immediate use.; Avoid repeated freezing and thawing.
• Shipping: Ambient