Bovine IgG (Fc specific) Rabbit Polyclonal Antibody

SKU R1305B

Bovine IgG (Fc specific) rabbit polyclonal antibody, Biotin

Product Data

• Application: ELISA, IF, IHC, WB
• Recommended Dilution: Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.; Note: This product has been assayed against 1.0 ug of Bovine IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/2,000 to 1/15,000 of the reconstitution concentration is suggested for this product.; Recommended Dilutions:; ELISA: 1/20,000-1/100,000; Western Blot: 1/2,000-1/10,000; Immunohistochemistry: 1/1,000-1/5,000
• Reactivity: Bovine
• Antibody Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Bovine IgG F(c) fragment.
• Specificity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Bovine IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.; Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, Bovine IgG, Bovine IgG F(c) and Bovine Serum.; No reaction was observed against Bovine IgG F(ab')2.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/ml Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer and 0.01% (w/v) sodium azide as preservative.; Label: Biotin; State: Lyophilized purified Ig fraction.; Molar radio: 10-20 BAC molecules per Rabbit IgG molecule.
• Reconstitution Method: Restore with 1.0 ml of deionized water (or equivalent).
• Concentration: lot specific
• Purification: Immunoaffinity chromatography.
• Conjugation: Biotin
• Storage: Store vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature.; This antibody is stable for one month at 2-8°C as an undiluted liquid.; Dilute only prior to immediate use.; Avoid repeated freezing and thawing.
• Shipping: Ambient