Chicken IgG / Chicken IgY (H+L chain), adsorbed Goat Polyclonal Antibody
Product Data | |
Recommended Dilution | Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. |
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Reactivity | Chicken |
Antibody Host | Goat |
Clonality | Polyclonal |
Immunogen | Chicken IgG whole molecule |
Specificity | Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Chicken IgG and Chicken Serum. No reaction was observed against Bovine, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit, Rat and Sheep Serum Proteins. |
Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 10 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free as a stabilizer and 0.01% (w/v) Sodium Azide as preservative Label: TRITC State: Lyophilized Ig fraction Label: Tetramethylrhodamine isothiocyanante (Molecular Weight 444 daltons) Absorption emission: 550 nm / 570 nm Molar radio: 3.5 moles TRITC per mole of Goat IgG |
Reconstitution Method | Restore with 1.0 ml of deionized water (or equivalent) |
Concentration | lot specific |
Purification | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. |
Conjugation | TRITC |
Storage | Store vial at 4°C prior to restoration. Restore with deionized water or equivalent; centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. For extended storage mix product with glycerol to 50% and then aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use. |
Shipping | Ambient |
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