Mouse IgG (H+L chain) Rabbit Polyclonal Antibody

SKU R1253T

Mouse IgG (H+L chain) rabbit polyclonal antibody, TRITC

Product Data

• Application: ELISA, IF
• Recommended Dilution: Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.; Recommended Dilution(s):; FLISA : 1:10,000 - 1:50,000; IF Microscopy : 1:1,000 - 1:5,000; Other Dilution: FLOW CYTOMETRY 1:500 - 1:2,500
• Reactivity: Mouse
• Antibody Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Mouse IgG whole molecule.
• Specificity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.; Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Mouse IgG and Mouse Serum.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 10 mg/ml BSA (IgG and Protease free) as stabilizer and 0.01% (w/v) Sodium Azide as preservative.; Label: TRITC; State: Lyophilzed purified IgG fraction; Label: Tetramethylrhodamine isothiocyanante (Molecular Weight 444 daltons); Absorption emission: 550 nm / 570 nm; Molar radio: 3.0 moles TRITC per mole of Rabbit IgG
• Reconstitution Method: Reconstitute with 1.0 ml of deionized water or equivalent.
• Concentration: lot specific
• Purification: Immunoaffinity Chromatography.
• Conjugation: TRITC
• Storage: Store vial at 2-8°C prior to restoration. Centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8°C as an undiluted liquid.; For extended storage aliquot contents and freeze at -20°C or below.; Avoid repeated freezing and thawing.; Dilute only prior to immediate use.
• Shipping: Blue Ice