CD4 Mouse Monoclonal Antibody [Clone ID: UMAB64]

CAT#: UM800010CF

Carrier-free (BSA/glycerol-free) CD4 mouse monoclonal antibody,clone UMAB64

Product Images

Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-CD4 mouse monoclonal antibody. (UM800010; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-CD4 mouse monoclonal antibody. (UM800010; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded human tonsil using anti-CD4 clone UMAB64 mouse monoclonal antibody at 1:200 dilution of 1mg/mL and detection with Polink2 Broad HRP DAB. UM800010 requires heat-induced epitope retrieval with Accel for 3minutes at110C in pressure chamber. The image shows the germinal and non germinal cells have weak to medium cytoplasmic and memeranous staining.

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  Western Blot analysis of HL-60 and SW-620 cell lysates (35ug) by using anti-CD4 monoclonal antibody (Clone UMAB64)

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  Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Colon, 9: Spleen, 10: Thyroid). Diluation: 1:500.

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  Immunofluorescent staining of Jurkat cells using anti-CD4 mouse monoclonal antibody (UM800010, green, 1:50). Actin filaments were labeled with Alexa Fluor® 594 Phalloidin (red), and nuclear with DAPI (blue). Scale bar, 4µm.

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Specifications

Product Data

• Clone Name: UMAB64
• Applications: IF, IHC, WB
• Recommended Dilution: IHC 1:50~100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CD4 (NP_000607) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 48.3 kDa
• Gene Name: CD4 molecule
• Database Link:
  NP_000607
  Entrez Gene 920 Human
  UniProt ID P01730
• Background: This gene encodes a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigenes and is also a receptor for the human immunodeficiency virus. This gene is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene. [provided by RefSeq, Aug 2010]
• Synonyms: CD4mut; IMD79; OKT4D

Reference Data

• Protein Families: Adult stem cells, Druggable Genome, ES Cell Differentiation/IPS, Induced pluripotent stem cells, Transmembrane
• Protein Pathways: Antigen processing and presentation, Cell adhesion molecules (CAMs), Hematopoietic cell lineage, Primary immunodeficiency, T cell receptor signaling pathway