GFP Rabbit Polyclonal Antibody

CAT#: TP401

GFP rabbit polyclonal antibody

Product Images

Lineage tracing of EGFP expressing migrating pectoral girdle myogenic precursors in the chicken embryos via electroporation using TOL-2-GFP plasmid system. Vibratome sections stained with anti-GFP antibody Cat.-No. TP401 show the labeled muscle fiber soft hepectoral girdle in chicken embryos at stage HH27. Data was kindly provided by: Nargis Khalida, Rizwan Rehimi: Institute of Anatomy, Dept. of Molecular Embryology, Ruhr University Bochum, Germany

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  Western blot detection of GFP-MIP2 fusion protein in transfectant by anti-GFP antibody.

Specifications

Product Data

• Applications: ELISA, IP, WB
• Recommended Dilution: ELISA.
  Western blot: 1/5,000.
  Immunoprecipitation: 1/200-1/500.
  The antibody has successfully been used in Immunohistochemistry on Vibratome sections and in Filter Binding Assays.
• Reactivities: A. victoria
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: E.coli expressed full-length GFP (Green Fluorescent Protein).
• Specificity: This antibody reacts with wild-type GFP, and its variants such as EGFP and EBFP.
• Formulation: State: Purified
  State: Lyophilized purified IgG fraction
  Preservative: 0.09% Sodium Azide
• Reconstitution Method: Restore to 1 mg/ml by adding 0.2 ml water.
• Purification: Protein A Chromatography
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month
  or (in small aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
  Shelf life: one year from despatch
• Background: Green fluorescent protein (GFP) is a spontaneously fluorescent protein isolated from the Pacific jellyfish, Aequorea victoria. It transduces the blue chemiluminescence into green fluorescent light. Since the molecular cloning of GFP cDNA1 and demonstration that GFP can be expressed as a functional transgene2, GFP has become a new tool with exciting applications in developmental, cell and molecular biology3. GFP is an ideal fluorescent probe: its fluorescence is not species specific and can be expressed in bacteria, yeast, plant and mammalian cells; it can fuse with proteins of interest without interfering significantly with their assembly or function.
• Synonyms: Green fluorescent protein, GFP-Tag
• Note: This product was originally produced by Torrey Pine Biolabs