ALKBH5 Mouse Monoclonal Antibody [Clone ID: OTI5B4]

SKU TA815646S

ALKBH5 mouse monoclonal antibody,clone OTI5B4

Product Data

• Clone Name: OTI5B4
• Application: WB
• Recommended Dilution: WB 1:5000
• Reactivity: Human, Mouse
• Antibody Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment of Human ALKBH5 (NP_060228) produced in Ecoli.
• Buffer: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Shipped at -20°C or with ice packs, Upon delivery store at -20°C. Dilute in PBS(pH7.3) if necessary. Stable for 12 months from date of receipt. Avoid repeated freeze-thaws.
• Stability: Stable for 12 months from date of receipt.
• Shipping: Blue Ice
• Predicted Protein Size: 51.4KD
• Gene Name: alkB homolog 5, RNA demethylase
• Database Link:
  NP_060228
  Q6P6C2
• Background: Dioxygenase that demethylates RNA by oxidative demethylation: specifically demethylates N(6)-methyladenosine (m6A) RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes (PubMed:23177736, PubMed:24489119, PubMed:24616105, PubMed:24778178). Can also demethylate N(6)-methyladenosine in single-stranded DNA (in vitro) (PubMed:24616105). Requires molecular oxygen, alpha-ketoglutarate and iron (PubMed:21264265, PubMed:23177736, PubMed:24489119, PubMed:24616105, PubMed:24778178). Demethylation of m6A mRNA affects mRNA processing and export (PubMed:23177736). Required for the late meiotic and haploid phases of spermatogenesis by mediating m6A demethylation in spermatocytes and round spermatids: m6A demethylation of target transcripts is required for correct splicing and the production of longer 3' UTR mRNAs in male germ cells (By similarity).[UniProtKB/Swiss-Prot Function]
• Synonyms: ABH5; OFOXD; OFOXD1

Reference Data