Transglutaminase 2 (TGM2) Mouse Monoclonal Antibody [Clone ID: OTI4H5]

CAT#: TA809245

TGM2 mouse monoclonal antibody,clone OTI4H5

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY TGM2 (Cat# RC217353, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TGM2 (Cat# TA809245). Positive lysates LY401458 (100ug) and LC401458 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 4 different cell lines by using anti-TGM2 monoclonal antibody (1:500).

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue tissue using anti-TGM2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809245) (1:1000)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-TGM2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809245) (1:1000)

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  Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-TGM2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809245) (1:1000)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-TGM2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809245) (1:1000)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-TGM2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809245) (1:1000)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-TGM2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809245) This figure shows negative staining (1:1000).

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-TGM2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809245) (1:1000)

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Specifications

Product Data

• Clone Name: OTI4H5
• Applications: IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:1000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human TGM2 (NP_004604) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 77.1 kDa
• Gene Name: transglutaminase 2
• Database Link:
  NP_004604
  Entrez Gene 7052 Human
  UniProt ID P21980
• Background: Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon-gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. The protein encoded by this gene acts as a monomer, is induced by retinoic acid, and appears to be involved in apoptosis. Finally, the encoded protein is the autoantigen implicated in celiac disease. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
• Synonyms: G-ALPHA-h; GNAH; HEL-S-45; TG2; TGC

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Huntington's disease