Osteopontin (SPP1) Mouse Monoclonal Antibody [Clone ID: OTI5E4]

SKU TA806784

SPP1 mouse monoclonal antibody, clone OTI5E4 (formerly 5E4)

Product Data

• Clone Name: OTI5E4
• Application: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivity: Human
• Antibody Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 17-314 of human SPP1(NP_001035147) produced in E.coli.
• Buffer: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Shipping: Blue Ice
• Predicted Protein Size: 33.7 kDa
• Gene Name: secreted phosphoprotein 1
• Database Link:
  NP_001035147
  P10451
• Background: The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
• Synonyms: BNSP; BSPI; ETA-1; OPN

Reference Data

• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: ECM-receptor interaction, Focal adhesion, Toll-like receptor signaling pathway