Galectin 3 (LGALS3) Mouse Monoclonal Antibody [Clone ID: OTI2F1]

CAT#: TA804567

LGALS3 mouse monoclonal antibody, clone OTI2F1 (formerly 2F1)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LGALS3 (RC208785, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LGALS3. Positive lysates LY400834 (100ug) and LC400834 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-LGALS3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804567)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-LGALS3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804567)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-LGALS3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804567)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-LGALS3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804567)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-LGALS3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804567)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-LGALS3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804567)

• Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-LGALS3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804567)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-LGALS3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804567)

• Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-LGALS3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804567)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-LGALS3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804567)

• Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-LGALS3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804567)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-LGALS3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804567)

• Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-LGALS3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804567)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-LGALS3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804567)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-LGALS3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804567)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-LGALS3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804567)

• Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-LGALS3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804567)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-LGALS3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804567)

• Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-LGALS3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804567)

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-LGALS3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804567)

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-LGALS3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804567)

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Specifications

Product Data

• Clone Name: OTI15E4 OTI1A1 OTI1A10 OTI1A2 OTI1A3 OTI1A4 OTI1C7 OTI1G8 OTI21C1 OTI21D10 OTI23D12 OTI2F1 OTI4A7 OTI6E1 OTI7G9 OTI8D7 OTI8E3 OTI9C7
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human LGALS3 (NP_002297) produced in E.coli.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 26 kDa
• Gene Name: lectin, galactoside binding soluble 3
• Database Link:
  NP_002297
  Entrez Gene 16854 MouseEntrez Gene 3958 Human
  UniProt ID P17931
• Background: This gene encodes a member of the galectin family of carbohydrate binding proteins. Members of this protein family have an affinity for beta-galactosides. The encoded protein is characterized by an N-terminal proline-rich tandem repeat domain and a single C-terminal carbohydrate recognition domain. This protein can self-associate through the N-terminal domain allowing it to bind to multivalent saccharide ligands. This protein localizes to the extracellular matrix, the cytoplasm and the nucleus. This protein plays a role in numerous cellular functions including apoptosis, innate immunity, cell adhesion and T-cell regulation. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Apr 2010]
• Synonyms: CBP35; GAL3; GALBP; GALIG; L31; LGALS2; MAC2

Reference Data

• Protein Families: Secreted Protein