GLI2 Mouse Monoclonal Antibody [Clone ID: OTI1F9]

CAT#: TA804549S

GLI2 mouse monoclonal antibody, clone OTI1F9 (formerly 1F9)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY GLI2 (Cat# RC217291, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GLI2(Cat# TA804549). Positive lysates LY417413 (100ug) and LC417413 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human skin tissue using anti-GLI2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804549)

Specifications

Product Data

• Clone Name: OTI1F9
• Applications: IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:150
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 729-1123 of human GLI2 (NP_005261) produced in E.coli.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 167.6 kDa
• Gene Name: GLI family zinc finger 2
• Database Link:
  NP_005261
  Entrez Gene 14633 MouseEntrez Gene 2736 Human
  UniProt ID P10070
• Background: This gene encodes a protein which belongs to the C2H2-type zinc finger protein subclass of the Gli family. Members of this subclass are characterized as transcription factors which bind DNA through zinc finger motifs. These motifs contain conserved H-C links. Gli family zinc finger proteins are mediators of Sonic hedgehog (Shh) signaling and they are implicated as potent oncogenes in the embryonal carcinoma cell. The protein encoded by this gene localizes to the cytoplasm and activates patched Drosophila homolog (PTCH) gene expression. It is also thought to play a role during embryogenesis. The encoded protein is associated with several phenotypes- Greig cephalopolysyndactyly syndrome, Pallister-Hall syndrome, preaxial polydactyly type IV, postaxial polydactyly types A1 and B. [provided by RefSeq, Jul 2008]
• Synonyms: CJS; HPE9; PHS2; THP1; THP2

Reference Data

• Protein Families: Adult stem cells, Cancer stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS
• Protein Pathways: Basal cell carcinoma, Hedgehog signaling pathway, Pathways in cancer