Vimentin (VIM) Mouse Monoclonal Antibody [Clone ID: OTI5D7]

CAT#: TA801250

VIM mouse monoclonal antibody, clone OTI5D7 (formerly 5D7)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VIM (RC201546, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VIM. Positive lysates LY401165 (100ug) and LC401165 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from 1 cell line by using anti-VIM monoclonal antibody at 1:200.

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  Western blot analysis of extracts (35ug) from 4 different cell lines by using anti-VIM monoclonal antibody (1:500).

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  Western blot analysis of extracts (35ug) from 2 different cell lines by using anti-VIM monoclonal antibody (1:500).

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type Hela cells (WT, Cat# LC810HELA) and VIM-Knockout Hela cells (KO, Cat# LC810257) were separated by SDS-PAGE and immunoblotted with anti-VIM monoclonal antibody TA801250, (1:500). Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody (TA500494) as a loading control.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-VIM mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA801250)

• Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-VIM mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA801250)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-VIM mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA801250)

• Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-VIM mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA801250)

• Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-VIM mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA801250)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-VIM mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA801250)

• Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-VIM mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA801250)

• Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-VIM mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA801250)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-VIM mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA801250)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-VIM mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA801250)

• Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-VIM mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA801250)

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-VIM mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA801250)

• Immunofluorescent staining of Hela cells using anti-VIM mouse monoclonal antibody (TA801250, green, upper left; merged, upper right) or Isotype control ( merged, lower right). Cell nuclei were stained with DAPI (blue, lower left) (1:100).

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Specifications

Product Data

• Clone Name: OTI5D7
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:200 - 1:1000, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 210-466 of human VIM (NP_003371) produced in E.coli.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 53.5 kDa
• Gene Name: vimentin
• Database Link:
  NP_003371
  Entrez Gene 22352 MouseEntrez Gene 81818 RatEntrez Gene 7431 Human
  UniProt ID P08670
• Background: This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract. [provided by RefSeq, Jun 2009]
• Synonyms: CTRCT30; HEL113

Reference Data

• Protein Families: ES Cell Differentiation/IPS