Vimentin (VIM) Mouse Monoclonal Antibody [Clone ID: OTI5D7]
SKU
CF801250
Carrier-free (BSA/glycerol-free) VIM mouse monoclonal antibody, clone OTI5D7 (formerly 5D7)
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TrueMAB™
Antibodies made against full-length proteins as antigens.
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Product Data | |
Clone Name | OTI5D7 |
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Application | IF, IHC, WB |
Recommended Dilution | WB 1:200 - 1:1000, IHC 1:150 |
Reactivity | Human, Mouse, Rat |
Antibody Host | Mouse |
Isotype | IgG2a |
Clonality | Monoclonal |
Immunogen | Human recombinant protein fragment corresponding to amino acids 210-466 of human VIM (NP_003371) produced in E.coli. |
Buffer | Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose) |
Reconstitution Method | For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific) |
Purification | Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G) |
Conjugation | Unconjugated |
Storage | Store at -20°C as received. |
Stability | Stable for 12 months from date of receipt. |
Shipping | Ambient |
Predicted Protein Size | 53.5 kDa |
Gene Name | vimentin |
Database Link | |
Background | This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract. [provided by RefSeq, Jun 2009] |
Synonyms | CTRCT30; HEL113 |
Reference Data | |
Protein Families | ES Cell Differentiation/IPS |
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Antibody Resources |
SKU | Description | Size | Price | |
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TA600390 | VIM mouse monoclonal capture antibody, validated for ELISA and Luminex assays | 100 ug |
$458.00
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TA801250 | VIM mouse monoclonal antibody, clone OTI5D7 (formerly 5D7) | 100 ul |
$447.00
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TA801250AM | VIM mouse monoclonal antibody,clone 5D7, Biotinylated | 100 ul |
$509.00
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TA801250BM | VIM mouse monoclonal antibody,clone 5D7, HRP conjugated | 100 ul |
$509.00
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TA801250S | VIM mouse monoclonal antibody, clone OTI5D7 (formerly 5D7) | 30 ul |
$200.00
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Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.