Vimentin (VIM) Biotinylated Mouse Monoclonal Detection Antibody (Biotin conjugated) [Clone ID: OTI7B10]

SKU TA700390

VIM biotinylated mouse monoclonal antibody, clone OTI7B10

Product Data

• Clone Name: OTI7B10
• Application: ELISA
• Reactivity: Human
• Antibody Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human VIM (NP_003371) produced in E.coli.
• Buffer: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Shipping: Blue Ice
• Predicted Protein Size: 53.5 kDa
• Gene Name: vimentin
• Database Link:
  NP_003371
  P08670
• Background: Vimentin (VIM) is a structural protein that in humans is encoded by the VIM gene. A vimentin monomer has a central α-helical domain, capped on each end by non-helical amino (head) and carboxyl (tail) domains. Two monomers are likely co-translationally expressed in a way that facilitates their formation of a coiled-coil dimer, which is the basic subunit of vimentin assembly. It is a type III intermediate filament (IF) protein that is expressed in mesenchymal cells. Intermediate filaments, along with tubulin-based microtubules and actin-based microfilaments, comprises the cytoskeleton. Vimentin is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. Since vimentin is the major cytoskeletal component of mesenchymal cells, it is often used as a marker of mesenchymally-derived cells or cells undergoing an epithelial-to-mesenchymal transition during both normal development and metastatic progression. Vimentin has been used as an immunohistochemical tumor marker of sarcoma, renal cell carcinoma, endometrial cancer, lung carcinoma, lymphoma, leukemia, and melanoma.

Reference Data

• Protein Families: ES Cell Differentiation/IPS