PD-L1 (CD274) Rabbit Monoclonal Antibody [Clone ID: OR-5E3]

CAT#: TA591004

Rabbit monoclonal anti-PD-L1 Antibody, clone OR-5E3

Product Images

Immunohistochemical staining of FFPE mouse spleen with Rb monoclonal PD-L1 C/N (TA591004, clone OR-5E3) at 0.9mg/mL at 1:300. Rb anti-PD-L1 clone OR-5E3 requires heat-induced epitope retrieval (HIER) at 120°C for 3min with TE (EDTA-Tris pH9 ). Detection was done with D13 Polink1 polymer detection system and visualized with DAB.

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  Immunohistochemical staining of FFPE mouse colon with Rb monoclonal PD-L1 C/N (TA591004, clone OR-5E3) at 0.9mg/mL at 1:300. Rb anti-PD-L1 clone OR-5E3 requires heat-induced epitope retrieval (HIER) at 120°C for 3min with TE (EDTA-Tris pH9 ). Detection was done with D13 Polink1 polymer detection system and visualized with DAB.

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  Immunohistochemical staining of FFPE human placenta with Rb monoclonal PD-L1 C/N TA591004 clone OR-5E3 at 1:100. Rb anti-PD-L1 clone OR-5E3 requires heat-induced epitope retrieval (HIER) at 120°C for 3min with ACCEL (EDTA-Tris pH8.7). Detection was done with Polink2 polymer detection system and visualized with DAB. Strong membrane staining seen in trophoblasts.

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  Immunohistochemical staining of FFPE human melanoma with Rb monoclonal PD-L1 C/N TA591004 clone OR-5E3 at 1:100. Rb anti-PD-L1 clone OR-5E3 requires heat-induced epitope retrieval (HIER) at 120°C for 3min with ACCEL (EDTA-Tris pH8.7). Detection was done with Polink2 polymer detection system and visualized with DAB. Strong membrane staining seen in tumor cells.

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  Immunohistochemical staining of FFPE human lung cancer case 1 with Rb monoclonal PD-L1 C/N TA591004 clone OR-5E3 at 1:100. Rb anti-PD-L1 clone OR-5E3 requires heat-induced epitope retrieval (HIER) at 120°C for 3min with ACCEL (EDTA-Tris pH8.7). Detection was done with Polink2 polymer detection system and visualized with DAB. Strong membrane staining seen in tumor cells.

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  Immunohistochemical staining of FFPE human lung cancer case 2 with Rb monoclonal PD-L1 C/N TA591004 clone OR-5E3 at 1:100. Rb anti-PD-L1 clone OR-5E3 requires heat-induced epitope retrieval (HIER) at 120°C for 3min with ACCEL (EDTA-Tris pH8.7). Detection was done with Polink2 polymer detection system and visualized with DAB. Strong membrane staining seen in tumor cells.

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Specifications

Product Data

• Clone Name: OR-5E3
• Applications: IHC
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: Synthetic peptide within Human PD-L1 C-terminal. The exact sequence is proprietary.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 34 kDa
• Gene Name: CD274 molecule
• Database Link:
  NP_054862
  Entrez Gene 60533 MouseEntrez Gene 29126 Human
  UniProt ID Q9NZQ7
• Background: This gene encodes an immune inhibitory receptor ligand that is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. The encoded protein is a type I transmembrane protein that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Expression of this gene in tumor cells is considered to be prognostic in many types of human malignancies, including colon cancer and renal cell carcinoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]
• Synonyms: B7-H; B7H1; PD-L1; PDCD1L1; PDCD1LG1; PDL1

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Cell adhesion molecules (CAMs)