PADI4 Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI4H5]

CAT#: TA504813AM

PADI4 mouse monoclonal antibody, clone OTI4H5 (formerly 4H5), Biotinylated

Product Images

Western blot analysis of overexpressed lysates(15ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, lane 1) , human PADI4 plasmid (RC206501, lane 2), mouse ALOX15 plasmid (MR216758, lane 3) using anti-ALOX15 antibody TA504813 (1:500).

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  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-PADI4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504813).

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-PADI4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504813).

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PADI4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504813).

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PADI4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504813).

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PADI4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504813).

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-PADI4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504813).

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PADI4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504813).

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PADI4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504813).

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-PADI4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504813).

• Anti-PADI4 mouse monoclonal antibody (TA504813) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PADI4 (RC206501).

• Flow cytometric Analysis of Hela cells, using anti-PADI4 antibody (TA504813), (Red), compared to a nonspecific negative control antibody, (Blue).

• Flow cytometric Analysis of Jurkat cells, using anti-PADI4 antibody (TA504813), (Red), compared to a nonspecific negative control antibody, (Blue).

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Specifications

Product Data

• Clone Name: OTI4H5
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PADI4(NP_036519) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 73.9 kDa
• Gene Name: peptidyl arginine deiminase 4
• Database Link:
  NP_036519
  Entrez Gene 18602 MouseEntrez Gene 29512 RatEntrez Gene 23569 Human
  UniProt ID Q9UM07
• Background: This gene is a member of a gene family which encodes enzymes responsible for the conversion of arginine residues to citrulline residues. This gene may play a role in granulocyte and macrophage development leading to inflammation and immune response. [provided by RefSeq]
• Synonyms: PAD; PAD4; PADI5; PDI4; PDI5