LOX Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI6B7]

CAT#: TA500869AM

LOX mouse monoclonal antibody, clone OTI6B7 (formerly 6B7), Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LOX (RC213323, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LOX. Positive lysates LY400843 (100ug) and LC400843 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from 1 cell line by using anti-LOX monoclonal antibody (1:200).

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-LOX mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500869)

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  Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-LOX mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500869)

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  Anti-LOX mouse monoclonal antibody (TA500869) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LOX (RC213323).

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  HEK293T cells transfected with either RC213323 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-LOX antibody (TA500869), and then analyzed by flow cytometry.

• Flow cytometric Analysis of Hela cells, using anti-LOX antibody (TA500869), (Red), compared to a nonspecific negative control antibody, (Blue).

• Flow cytometric Analysis of Jurkat cells, using anti-LOX antibody (TA500869), (Red), compared to a nonspecific negative control antibody, (Blue).

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Specifications

Product Data

• Clone Name: OTI6B7
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:1000, IHC 1:50, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 22-168 of human LOX (NP_002308) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 46.8 kDa
• Gene Name: lysyl oxidase
• Database Link:
  NP_002308
  Entrez Gene 16948 MouseEntrez Gene 24914 RatEntrez Gene 4015 Human
  UniProt ID P28300
• Background: The protein encoded by this gene is an extracellular copper enzyme that initiates the crosslinking of collagens and elastin. The enzyme catalyzes oxidative deamination of the epsilon-amino group in certain lysine and hydroxylysine residues of collagens and lysine residues of elastin. In addition to crosslinking extracellular matrix proteins, the encoded protein may have a role in tumor suppression. Defects in this gene are a cause of autosomal recessive cutis laxa type I (CL type I). Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
• Synonyms: MGC105112

Reference Data

• Protein Families: Druggable Genome, Secreted Protein