Alpha B Crystallin (CRYAB) Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI6D11]

CAT#: TA500583AM

CRYAB (Alpha B Crystallin) mouse monoclonal antibody, clone OTI6D11 (formerly 6D11), Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY CRYAB (Cat# RC202718, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CRYAB (Cat# TA500583). Positive lysates LY419682 (100ug) and LC419682 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-CRYAB monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-CRYAB monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-CRYAB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500583)

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  Immunohistochemical staining of paraffin-embedded human smooth muscle cancer tissue using anti-CRYAB mouse monoclonal antibody. (Heat-induced epitope retrieval, TA500583). Data courtesy of a collaborative pathologist.

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  Anti-CRYAB mouse monoclonal antibody (TA500583) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CRYAB (RC202718).

• Immunofluorescent staining of HT29 cells using anti-CRYAB mouse monoclonal antibody (TA500583).

• Immunoprecipitation (IP) of CRYAB by using TrueMab monoclonal anti-CRYAB antibodies (Negative control: IP without adding anti-CRYAB antibody.). For each experiment, 500ul of DDK tagged CRYAB overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-CRYAB antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

• HEK293T cells transfected with either RC202718 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CRYAB antibody (TA500583), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI6D11
• Applications: FC, IF, IHC, IP, WB
• Recommended Dilution: WB 1:500~1000, IHC 1:50, IF 1:50~100, FLOW 1:100, IP 2ug/500ul
• Reactivities: Human, Rat, Monkey, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CRYAB (NP_001876) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 20 kDa
• Gene Name: crystallin alpha B
• Database Link:
  NP_001876
  Entrez Gene 12955 MouseEntrez Gene 25420 RatEntrez Gene 710747 MonkeyEntrez Gene 1410 Human
  UniProt ID P02511
• Background: Crystallins are separated into two classes: taxon-specific, or enzyme, and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. Since lens central fiber cells lose their nuclei during development, these crystallins are made and then retained throughout life, making them extremely stable proteins. Mammalian lens crystallins are divided into alpha, beta, and gamma families; beta and gamma crystallins are also considered as a superfamily. Alpha and beta families are further divided into acidic and basic groups. Seven protein regions exist in crystallins: four homologous motifs, a connecting peptide, and N- and C-terminal extensions. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (sHSP also known as the HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. Post-translational modifications decrease the ability to chaperone. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. Alpha-A and alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. Elevated expression of alpha-B crystallin occurs in many neurological diseases; a missense mutation cosegregated in a family with a desmin-related myopathy. [provided by RefSeq]
• Synonyms: CMD1II; CRYA2; CTPP2; CTRCT16; HEL-S-101; HSPB5; MFM2