ICAD (DFFA) Mouse Monoclonal Antibody [Clone ID: OTI3F12]

SKU TA500063

DFF45 (ICAD) mouse monoclonal antibody, clone OTI3F12 (formerly 3F12)

Product Data

• Clone Name: OTI3F12
• Application: IF, WB
• Recommended Dilution: WB 1:200~500, IF 1:100
• Reactivity: Human, Monkey
• Antibody Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human DFFA (NP_004392) produced in E.coli.
• Buffer: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.2 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Shipping: Blue Ice
• Predicted Protein Size: 36.3 kDa
• Gene Name: DNA fragmentation factor subunit alpha
• Database Link:
  NP_004392
  O00273
• Background: Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
• Synonyms: DFF-45; DFF1; ICAD

Reference Data

• Protein Pathways: Apoptosis