BRD4 Rabbit Monoclonal Antibody
Product Data | |
Application | ICC/IF, IHC, IP, WB |
---|---|
Recommended Dilution | WB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:50 |
Reactivity | Human, Mouse, Rat |
Antibody Host | Rabbit |
Isotype | IgG |
Clonality | Monoclonal |
Immunogen | A synthesized peptide derived from human Brd4 |
Specificity | Brd4 Antibody detects endogenous levels of total Brd4 |
Buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Concentration | lot specific |
Purification | Affinity-chromatography |
Conjugation | Unconjugated |
Storage | Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle. |
Shipping | Blue Ice |
Predicted Protein Size | 152kDa |
Gene Name | bromodomain containing 4 |
Database Link | |
Background | Bromodomain-containing protein 4 (BRD4) is a member of the bromodomains and extra terminal (BET) family of proteins, which also includes BRD2, BRD3, and BRDT. BET family proteins contain two tandem bromodomains and an extra terminal (ET) domain, and bind acetyl lysine residues. BRD4 is a chromatin-binding protein with a preference for Lys14 on histone H3 as well as Lys5 and Lys12 on histone H4. BRD4 chromatin binding occurs throughout the cell cycle, including condensed mitotic chromosomes, when the majority of genes are silenced. |
Synonyms | Brd4; Bromodomain containing 4; CAP; chromosome associated protein; HUNK1; MCAP |
Reference Data |
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