CH25H Rabbit Polyclonal Antibody

SKU TA397540S

CH25H Antibody

Product Data

• Application: ELISA, IHC, WB
• Recommended Dilution: WB: 1:1,000; IHC: 1:100; ELISA: 1:10,000 - 1:50,000
• Reactivity: Human
• Antibody Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Anti-CH25H antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-Terminal region of human CH25H conjugated to Keyhole Limpet Hemocyanin (KLH).
• Specificity: This affinity purified antibody is directed against human CH25H. This product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross-reactivity with the antigen based on 100% homology with the immunizing sequence and 92% homology to pig, 91% homology to rat, and 75% homology to mouse.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 0.92 mg/ml - lot specific
• Conjugation: Unconjugated
• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Stability: Expiration date is one (1) year from date of receipt.
• Shipping: Ambient
• Gene Name: cholesterol 25-hydroxylase
• Database Link:
  O95992
• Background: CH25H (Cholesterol 25-Hydroxylase catalyzes the formation of 25-hydroxycholesterol from cholesterol, leading to repress cholesterol biosynthetic enzymes. CH25H plays a key role in cell positioning and movement in lymphoid tissues: 25-hydroxycholesterol is an intermediate in biosynthesis of 7-alpha,25-dihydroxycholesterol (7-alpha,25-OHC), an oxysterol that acts as a ligand for the G protein-coupled receptor GPR183/EBI2, a chemotactic receptor for several lymphoid cells. It may play an important role in regulating lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein (SREBP) processing. In testis, production of 25-hydroxycholesterol by macrophages may play a role in Leydig cell differentiation. Anti-CH25H Antibody is useful for researchers interested in Alzheimer’s Disease, Neuroscience, and metabolism.
• Synonyms: Rabbit Anti-Cholesterol 25-Hydroxylase, Rabbit Anti-CH25H, Cholesterol 25-Monooxygenase, EC 1.14.99.38, H25OH, C25H
• Note: Anti-CH25H Antibody has been tested in ELISA, WB, and IHC. Expect a band at ~31.7kDa in western blot using appropriate tissues or lysates. Positive control used: Human Kidney Tissues in Immunohistochemistry.

Reference Data