H3C14 Rabbit Polyclonal Antibody

SKU TA397471

Histone H3 K4me3 Antibody

Product Data

• Application: ChIP, IF, IHC, WB
• Recommended Dilution: WB: 1:500; IHC: 1:500; IF: 1:500; CHiP: 2-5 µg/million cells
• Reactivity: C. elegans, Human, Mouse
• Antibody Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Histone H3 [Trimethyl Lys4] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic trimethylated peptide surrounding Lysine 4 of human Histone H3.2.
• Specificity: Anti-Histone H3 [Trimethyl Lys4] was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with human Histone H3.2. A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and C. elegans. Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant based on 100% sequence homology. Cross-reactivity with Histone H3 from other sources has not been determined.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 0.70 mg/ml - lot specific
• Conjugation: Unconjugated
• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Stability: Expiration date is one (1) year from date of receipt.
• Shipping: Ambient
• Gene Name: histone cluster 2, H3c
• Database Link:
  Q71DI3
• Background: H3K4Me3 is a common epigenetic modification that occurs in close proximity to nearly 2/3 of human gene promoters, and seems to balance another modification, H3K27Me3. These two modifications work in tandem to differentiate and/or maintain stem cells. Zygotic cells are activated in the most part by H3K4Me3; whereas, cancer is associated with disrupted associations between K3K4Me3 and ING1. Anti-Histone H3 are ideal for researchers interested in Chromatin Modifiers, Chromatin Research, Histones and Modified Histones, and Epigenetics research.
• Synonyms: rabbit anti-Histone H3 trimethyl Lys4 antibody, H3.3B, H3.3AH3F3H3F3B, H3 histone, family 3A, histone H3.3, MGC87783, MGC87782, H3K4me3
• Note: Anti-Histone H3 [Trimethyl Lys4] antibody is tested in Western Blot, Immunofluorescence, Chromatin Immunoprecipitation, and Dot Blot. This antibody is useful for Immunocytochemistry. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~15.4 kDa corresponding to Histone H3 protein by Western Blotting in HeLa histone prep lysate or the appropriate cell lysate or extract. Epi-Plus™ antibody production in collaboration with Novus Biologicals.

Reference Data

• Protein Pathways: Systemic lupus erythematosus