CPA1 Rabbit Polyclonal Antibody

SKU
TA397039
Carboxypeptidase A Antibody Biotin Conjugated
$825.00
5 Days*
Specifications
Product Data
Application ELISA, WB
Recommended Dilution WB: 1:500 - 1:3,000
ELISA: 1:2,000 - 1:10,000
Antibody Host Rabbit
Clonality Polyclonal
Immunogen Carboxypeptidase A [Bovine Pancreas]
Specificity Anti-Carboxypeptidase A is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Carboxypeptidase A [Bovine Pancreas]. Cross reactivity against Carboxypeptidase A from other sources may occur but have not been specifically determined.
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Method Restore with deionized water (or equivalent) - Reconstitution Volume: 100 µL
Concentration 1.0 mg/mL - lot specific
Conjugation Biotin
Storage Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Stability Expiration date is one (1) year from date of receipt.
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Database Link
Background Anti-Carboxypeptidase A antibody catalyzes the release of C-terminal amino acids with little or no action with –Asp, -Glu, -Arg, -Lys or –Pro. This antibody is ideal for investigators interested in Metabolism, Cancer, Signal Transduction, or Cell Biology research.
Synonyms rabbit anti-Carboxypeptidase A antibody biotin conjugation, biotin conjugated rabbit anti-Carboxypeptidase A Antibody, Carboxypeptidase A1 precursor antibody, CPA1 antibody, Pancreatic carboxypeptidase A1 antibody, Procarboxypeptidase A1 pancreatic antibody
Note Anti-Carboxypeptidase A Biotin Conjugated Antibody has been tested by ELISA and western blot and is assayed against 1.0 ug of Carboxypeptidase A in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:15,000 to 1:55,000 of the reconstitution concentration is suggested for this product.
Reference Data
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.