Rabbit Polyclonal Antibody

SKU TA392656

Smad2/3 (phospho-T8) polyclonal antibody

Product Data

• Application: WB
• Recommended Dilution: WB: 1:1000~1:2000
• Reactivity: Human, Mouse, Rat
• Antibody Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic phosphopeptide derived from human Smad2/3 around the phosphorylation site of Threonine 8.
• Specificity: p-Smad2/3 (T8) polyclonal antibody detects endogenous levels of Smad2/3 protein only when phosphorylated at Thr8.
• Buffer: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Concentration: 1mg/ml
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
• Stability: 1 year
• Shipping: Blue Ice
• Predicted Protein Size: ~ 55,60 kDa
• Database Link:
  P84022/Q15796
• Background: Smad proteins, the mammalian homologs of the Drosophila mothers against decapentaplegic (Mad), have been implicated as downstream effectors of GFβ/BMP signaling. Smad1 (also designated Madr1 or JV4-1) and Smad5 are effectors of BMP-2 and BMP-4 function, while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGFβ and activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to activin/TGFβ signaling by interfering with TGFβ-mediated phosphorylation of other Smad proteins.
• Synonyms: hMAD-2; hMAD-3; hSMAD2; hSMAD3; JV15-2; JV18-1; Mad-related protein 2; Mad3; MADH2; MADH3; MAD homolog 2; MAD homolog 3; MADR2; Mothers against decapentaplegic homolog 2; Mothers against decapentaplegic homolog 3; Mothers against DPP homolog 2; Mothers against DPP homolog 3; SMAD2; Smad2; SMAD 2; SMAD 3; SMAD3; Smad3; SMAD family member 2; SMAD family member 3
• Note: For research use only, not for use in diagnostic procedure.

Reference Data