Phospho-AKT1 (pThr342) Rabbit Polyclonal Antibody
Specifications
| Product Data | |
| Application | WB |
|---|---|
| Recommended Dilution | WB: 1:500 |
| Reactivity | Drosophila |
| Antibody Host | Rabbit |
| Isotype | IgG |
| Clonality | Polyclonal |
| Immunogen | Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr342 of Drosophila AKT, conjugated to keyhole limpet hemocyanin (KLH) |
| Specificity | Specific for endogenous levels of the ~68 kDa AKT protein phosphorylated at Thr342. Immunolabeling is completely eliminated with λ-phosphatase treatment. It has been reported that this antibody may also recognize some level of phosphorylated S6K as there is 67% homology with the sequence used as antigen. |
| Buffer | 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. |
| Concentration | lot specific |
| Purification | Antigen Affinity Purified from Pooled Serum |
| Conjugation | Unconjugated |
| Storage | Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
| Stability | After date of receipt, stable for at least 1 year at -20°C. |
| Shipping | Blue Ice |
| Predicted Protein Size | 68 |
| Background | The serine/threonine kinase Akt also known as protein kinase B (PKB) or Rac, plays a crucial role in controlling many diverse and important cellular functions such as cell survival and glycogen metabolism (Hajduch et al., 2001 and Nicholson & Anderson, 2002). Three isoforms (α, β, and γ) have been identified that can be activated rapidly in response to insulin and growth factors in a phosphoinositide 3-kinase (PI3K)-dependent fashion (Hajduch et al., 2003). Phosphorylation of Aktα occurs at two specific regulatory sites in Drosophila, one localized in the kinase domain, Thr342, and the other in the C-terminal regulatory domain, Ser505: these two activation sites are homologous to mammalian Ser473 and Thr308 respectively (Powell et al., 2004). |
| Note | Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns. |
| Reference Data | |
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
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