CD53 Rabbit Monoclonal Antibody [Clone ID: HD77]

SKU TA386337

Recombinant Anti-CD53 (Clone HD77)

Product Data

• Clone Name: HD77
• Application: FC, IF, IHC, IP, WB
• Reactivity: Human
• Antibody Host: Rabbit
• Isotype: IgG, kappa
• Clonality: Monoclonal
• Immunogen: This antibody was raised by immunising mice with human leukemia cells.
• Specificity: This antibody is specific for the extracytoplasmic part of human CD53, a pan-leukocyte antigen.; ; This antibody has applications in the detection of normal and neoplastic white blood cells. This antibody has been used to stain tumour and mononuclear cells for CD53 expression in flow cytometric analysis of samples from ovarian carcinoma patients (Marme et al, 2002), and cells of the colon carcinoma line HT29 (Koretz et al, 1992). In immunohistochemical analysis of frozen tissue sections, this antibody has been shown to react with osteoclasts and osteoclast-like giant cells in malignant fibrous histiocytoma and in benign giant cell tumor of tendon sheath (Mechtersheimer et al, 1990). This antibody has been used to re-immunoprecipitate CD53 from PKCα immunoprecipiates (Zhang et al, 2001).
• Buffer: PBS with 0.02% Proclin 300.
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Please store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. Avoid freeze and thaw cycles.
• Stability: 3 years from dispatch.
• Shipping: Blue Ice
• Gene Name: CD53 molecule
• Database Link:
  P19397
• Synonyms: MOX44; tetraspanin-25; Tspan-25; TSPAN25
• Note: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Reference Data