UBE2D3 Rabbit Polyclonal Antibody

CAT#: TA356287

UBE2D3 Antibody - N-terminal region

Product Images

WB Suggested Anti-UBE2D3 Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:1562500 Positive Control: MCF7 cell lysate.UBE2D3 is strongly supported by BioGPS gene expression data to be expressed in MCF7

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  Lanes: 1: 40ng HIS-UBE2D1 protein 2: 40ng HIS-UBE2D2 protein 3: 40ng HIS-UBE2D3 protein 4: 40ng HIS-UBE2D4 protein 5: 40ng HIS-UBE2E1 protein 6: 40ng HIS-UBE2E2 protein 7: 40ng HIS-UBE2E3 protein 8: 40ng HIS-UBE2K protein 9: 40ng HIS-UBE2L3 protein 10: 40ng HIS-UBE2N protein 11: 40ng HIS-UBE2V1 protein 12: 40ng HIS-UBE2V2 protein. Primary Antibody Dilution: 1:500 Secondary Antibody: Anti-rabbit-HRP Secondary Antibody Dilution: 1:50,000 Gene Name: UBE2D3 Submitted by: Dr Chris Boutell. MRC-UoG Centre for Virus Research (CVR), UK.

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  Host: Rabbit Target Name: UBE2D3 Sample Type: Jurkat Antibody Dilution: 1.0ug/mlUBE2D3 is strongly supported by BioGPS gene expression data to be expressed in Jurkat

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  Host: Rabbit Target Name: UBE2D3 Sample Type: MCF7 Antibody Dilution: 1.0ug/mlUBE2D3 is strongly supported by BioGPS gene expression data to be expressed in MCF7

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  Host: Rabbit Target Name: UBE2D3 Sample Type: Human Fetal Lung Antibody Dilution: 1.0ug/ml

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  Host: Rabbit Target Name: UBE2D3 Sample Type: Human Fetal Liver Antibody Dilution: 1.0ug/ml

• Host: Rabbit Target Name: UBE2D3 Sample Type: 293T Antibody Dilution: 1.0ug/mlUBE2D3 is strongly supported by BioGPS gene expression data to be expressed in Human HEK293T cells

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Specifications

Product Data

• Applications: WB
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human UBE2D3
• Specificity: Expected reactivity: Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Yeast, Zebrafish
  Homology: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 92%; Rat: 100%; Yeast: 92%; Zebrafish: 92%
• Formulation: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
  Note that this product is shipped as lyophilized powder to China customers.
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 17kDa
• Gene Name: ubiquitin conjugating enzyme E2 D3
• Database Link:
  NP_003331
  Entrez Gene 7323 Human
  UniProt ID P61077
• Background: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2D3 is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme functions in the ubiquitination of the tumor-suppressor protein p53, which is induced by an E3 ubiquitin-protein ligase.The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme functions in the ubiquitination of the tumor-suppressor protein p53, which is induced by an E3 ubiquitin-protein ligase. Multiple spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been determined.
• Synonyms: E2(17)KB3; MGC5416; MGC43926; UBC4/5; UBCH5C

Reference Data

• Protein Pathways: Ubiquitin mediated proteolysis