Cytokeratin 18 (KRT18) Rabbit Polyclonal Antibody

SKU TA354928

Rabbit anti CK-18 (Phospho) Polyclonal Antibody

Product Data

• Application: IHC, WB
• Recommended Dilution: WB 0.1-1 µg/ml ELISA 0.01-0.1 µg/ml IP 2-5 µg/ml IHC 2-10 µg/ml FC 5-10 µg/ml
• Reactivity: Human
• Antibody Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic peptide surrounding to the epitope -SAASVYA- with a phosphorylation site at Serine 33 of Cytokeratin 18 protein from human origin. It is identical among human, mouse, rat, etc..
• Buffer: This affinity purified antibody is supplied in sterile Phosphate-buffered saline (pH7.2) containing antibody stabilizer.
• Purification: The Rabbit IgG is purified by site-modified Epitope Affinity Purification.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Shipping: Blue Ice
• Predicted Protein Size: ~48 kDa
• Gene Name: keratin 18
• Database Link:
  NP_000215
  P05783
• Background: Keratin polypeptides 8 (basic keratin) and 18 (acidic keratin) exist in combination as K8/K18 which are the cytoskeletal intermediate filament proteins of hepatocytes while K8/K18/K19 are the keratins of hepatobiliary ductal cells. Hepatocyte K8/K18 are highly abundant and behave as stress proteins with injury-inducible expression. K8 and K18 are cytoprotective stress proteins that play a central role in guarding hepatocytes from apoptosis. Hepatocellular carcinoma have been reportedly defined by the use of antibodies that recognize only cytokeratins 8 and 18. K18 Ser33 as an interphase phosphorylation site, which increases its phosphorylation during mitosis in cultured cells and regenerating liver, and as an in vitro cdc2 kinase phosphorylation site.Phosphorylation of Ser33 and Ser52 are involved in keratin subcellular distribution.
• Synonyms: CYK18; K18

Reference Data

• Protein Pathways: Pathogenic Escherichia coli infection