Legumain (LGMN) Rabbit Polyclonal Antibody

SKU TA354520

Rabbit anti Legumain Polyclonal Antibody

Product Data

• Application: IHC, WB
• Recommended Dilution: WB 0.1-1 µg/ml ELISA 0.01-0.1 µg/ml IP 2-5 µg/ml IHC 2-10 µg/ml FC 5-10 µg/ml
• Reactivity: Human, Mouse, Rat
• Antibody Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic peptide corresponding to the internal sequence (a portion from 100aa-190aa) of human Legumain protein. This sequence is identical to mouse, human and rat.
• Buffer: This affinity purified antibody is supplied in sterile Phosphate buffered saline (pH7.2) containing antibody stabilizer.
• Purification: The Rabbit IgG is purified by Epitope Affinity Purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Shipping: Blue Ice
• Predicted Protein Size: ~50 kDa
• Gene Name: legumain
• Database Link:
  NP_001008530
  Q99538
• Background: Legumain, a 433 amino acid protein with 17 aa signal sequence, is a cystein protease that has a strict specificity for hydrolysis of asparaginyl bonds. This enzyme may be involved in the processing of bacterial peptides and endogenous proteins for MHC class II presentation in the lysosomal/endosomal systems. Enzyme activation is triggered by acidic pH and appears to be autocatalytic . Protein expression occurs after monocytes differentiate into dendritic cells. Overexpression of this gene may be associated with the majority of solid tumor types. This novel asparaginyl endopeptidase has been observed to be highly expressed in several types of tumors including colorectal cancer.
• Synonyms: AEP; LGMN1; PRSC1

Reference Data

• Protein Families: Druggable Genome, Protease
• Protein Pathways: Antigen processing and presentation, Lysosome