TNFAIP3 Mouse Monoclonal Antibody [Clone ID: 59A426]

SKU TA336834

Mouse Monoclonal A20/TNFAIP3 Antibody (59A426)

Product Data

• Clone Name: 59A426
• Application: CyTOF-ready, FC, ICC/IF, IHC, IP, Simple Western, WB
• Recommended Dilution: Flow Cytometry: 2.5 ug/ml, Immunohistochemistry-Paraffin: 5 - 10 ug/ml, Flow (Intracellular): 2.5 ug/ml, Western Blot: 2-4 ug/ml, Immunoprecipitation: 1-2 ug/ml, Immunocytochemistry/ Immunofluorescence: 10 ug/ml, Immunohistochemistry: 5 - 10 ug/ml, Simple Western: 1:100, CyTOF-ready
• Reactivity: Human, Mouse, Rat
• Antibody Host: Mouse
• Isotype: IgG1, kappa
• Clonality: Monoclonal
• Immunogen: Full length recombinant human A20. The epitope has been mapped to the C-terminal portion of A20 (amino acids 440-790)
• Buffer: PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at -20C. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Protein G purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Shipping: Blue Ice
• Gene Name: TNF alpha induced protein 3
• Database Link:
  NP_006281
  P21580
• Background: A20 (TNFAIP3) is an important regulator of proinflammatory signaling pathways, including NK-kB sginaling. A20 is upregulated through NF-kB (p50/p55) activation, and subsequently down regulates NF-kB through its dual function as a deubiquitinase and ubiquitin ligase (Evans, 2005). The A20 monoclonal antibody (clone 59A426) has been instrumental in discovering various nuances of NF-kB regulation and key to defining A20 biology.
• Synonyms: A20; OTUD7C; TNFA1P2
• Note: The A20 antibody (clone 59A426) has also been used in Immunohistochemistry-Paraffin (Metellus, 2010) and Flow Cytometry (intracellular) (Hjelmeland, 2010). Multiple A20 cleavage fragments have been described in Western Blot, see Coornaert (2008) and Hailfinger (2009) for additional details.

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: NOD-like receptor signaling pathway