Aurora A (AURKA) Rabbit Polyclonal Antibody

SKU TA336807

Rabbit Polyclonal Aurora A Antibody

Product Data

• Application: ICC/IF, IHC, Simple Western, WB
• Recommended Dilution: Western Blot: 1 - 2 ug/ml, Immunocytochemistry/ Immunofluorescence: 2 ug/ml, Immunohistochemistry: 1:200, Immunohistochemistry-Paraffin: 1:200, Simple Western: 1:200
• Reactivity: Human, Mouse
• Antibody Host: Rabbit
• Clonality: Polyclonal
• Immunogen: A genomic peptide made to an N-terminal region of the human Aurora A protein (within residues 50-200). [Swiss-Prot O14965]
• Buffer: PBS, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Immunogen affinity purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Shipping: Blue Ice
• Gene Name: aurora kinase A
• Database Link:
  NP_940835
  O14965
• Background: Aurora A is a cytoplasmic serine/threonine kinase that is critical in transitioning cells through mitosis and meiosis. Aurka acts through phosphorylating several spindle and tubulin proteins. Without Aurka, axon and neuron formation are negatively impacted. When these critical cellular processes are disrupted, there is a potential for tumorgenesis, and thus Aurka is implicated in many cancers, particularly those of the blood. In studies of angiofibromas, both MDM1 and Aurka were amplified. Overall, cell proliferation seems proportional to the expression of Aurka.
• Synonyms: AIK; ARK1; AURA; BTAK; PPP1R47; STK6; STK7; STK15
• Note: This AURKA antibody is useful for Immunohistochemistry and Western blot, where a band is seen ~45 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

Reference Data

• Protein Families: Druggable Genome, Protein Kinase, Stem cell - Pluripotency
• Protein Pathways: Oocyte meiosis