KDM3A / JHDM2A (KDM3A) Mouse Monoclonal Antibody [Clone ID: 1E12]

SKU TA336804

Mouse Monoclonal Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody (1E12)

Product Data

• Clone Name: 1E12
• Application: ELISA, ICC/IF, IHC, Simple Western, WB
• Recommended Dilution: Immunohistochemistry-Paraffin: 1:200-1:1000, Simple Western: 1:500, ELISA: 1:10000, Immunohistochemistry: 1:200-1:1000, Immunocytochemistry/ Immunofluorescence: 1:10-1:500, Western Blot: 1:500-1:2000
• Reactivity: Human
• Antibody Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of human JMJD1A expressed in E. coli. [UniProt# Q9Y4C1]
• Buffer: Preservative: 0.05% Sodium Azide. Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Ascites
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Shipping: Blue Ice
• Predicted Protein Size: 147 kDa
• Gene Name: lysine demethylase 3A
• Database Link:
  NP_001140160
  Q9Y4C1
• Background: JMJD1A is a histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. It preferentially demethylates mono- and dimethylated H3 'Lys-9' residue, with a preference for dimethylated residue, while it has weak or no activity on trimethylated H3 'Lys-9'. Demethylation of Lys residue generates formaldehyde and succinate. JMJD1A is involved in hormone-dependent transcriptional activation, by participating in recruitment to androgen-receptor target genes, resulting in H3 'Lys-9' demethylation and transcriptional activation. It's also Involved in spermatogenesis by regulating expression of target genes such as PRM1 and TMP1 which are required for packaging and condensation of sperm chromatin. Involved in obesity resistance through regulation of metabolic genes such as PPARA and UCP1.
• Synonyms: JHDM2A; JHMD2A; JMJD1; JMJD1A; TSGA
• Note: This JMJD1A (1E12) antibody is useful in Western Blot, ELISA and Immunohistochemistry on paraffin-embedded sections.

Reference Data